Detection of Blueberry red ringspot virus in different stages of Parthenolecanium corni in Poland
Abstract
Detection of Blueberry red ringspot virus in different stages of Parthenolecanium corni in Poland. European fruit lecanium scales (Parthenolecanium corni) appeared in a large mass on highbush blueberry plants in central Poland. Some of the highbush blueberry plants growing in the studied plantation were infected by Blueberry red ringspot virus (BRRSV). Since no insect vector of BRRV is known so far, attempts were made to establish whether the soft scale pests were able to uptake the virus with the sap of the infected plants during feeding. The virus was detected and identified using the PCR and the DNA-DNA dot blot hybridisation technique in almost all the tested larval stages (56/60) and adult females (18/20) of P. corni feeding on BRRSV-infected highbush blueberry plants.
Keywords
Blueberry red ringspot virus; Parthenolecanium corni; virus acquirement; Vaccinium corymbosum
References
BAHDE B.W., POOJARI S., ALABI O.J., NAIDU R.A., WALSH D.B. 2013: Pseudococcus maritimus (Hemiptera: Pseudococcidae) and Parthenolecanium corni (Hemiptera: Coccidae) are capable of transmitting Grapevine leafroll-associated virus 3 between Vitis ×labruscana and Vitis vinifera. Envirol. Entomol. 42 (6): 1292–1298.
BOOM R., SOL C.J.A., SALIMANS M.M.M., JANSEN C.L., WERTHEIM-Van DILLEN P.M.E., NORDAA van der J. 1990: Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28: 495–503.
CLINE W.O., BALLINGTON J.R., POLASHOCK J.J. 2009: Blueberry red ringspot observations and findings in North Carolina. Acta Hortic. (ISHS) 810: 305–312.
HERRBACH E., La MAGUET J., HOMMAY G. 2016: Virus transmission by mealybugs and soft scales (Hemiptera: Coccoidea). In: J.K. Brown (Ed.), Vector-mediated transmission of plant pathogens. APC Press, St. Paul, MN.
HOMMAY G., KOMAR V., LEMAIRE O., HERRBACH E. 2008: Grapevine virus A transmission by larvae of Parthenolecanium corni. Eur. J. Plant Pathol. 121 (2): 185–188.
International Committee on Taxonomy of Viruses – ICTV 2017: Virus Taxonomy: The ICTV Report on Virus Classification and Taxon Nomenclature. 10th ed. online. Available at: https://talk.ictvonline.org/ictv-reports/ictv_online_report
JEVREMOVIĆ D., PAUNOVIĆ S., LEPOSAVIĆ A. 2016: Incidence of viruses in highbush blueberry (Vaccinium corymbosum L.) in Serbia. Pestic. Phytomed. (Belgrade) 31 (1–2): 45–50.
MAROTTA S., TRANFAGLIA A. 1997: Biology. General life history. In: Y. Ben-Dov, C.J. Hodgson (Eds.), Soft scale insects – Their biology, natural enemies and control. Elsevier, Amsterdam: 251–225.
POLASHOCK J.J., EHLANFELDT M.K., CROUCH J.A. 2009: Molecular detection and discrimination of Blueberry red ringspot virus strains causing disease in cultivated blueberry and cranberry. Plant Dis. 93: 727–733.
SFORZA R., BOUDON-PADIEU E., GREIF, C. 2003: New mealybug species vectoring Grapevine leafroll associated viruses-1 and -3 (GLRaV-1 and -3). Eur. J. Plant Pathol. 109: 975–981.
