The use of tissue cultures in the mass production of Heuchera ‘Silver Scrolls’
Abstract
The use of tissue cultures in the mass production of Heuchera ‘Silver Scrolls’. Heuchera is one of the most important decorative perennials, present in garden, city and park plantings. In recent years, it has also gained importance as a plant for pot compositions, and as a floristic material. The aim of this work is to present an effective protocol for the intensification of the production of Heuchera ‘Silver Scrolls’ in tissue cultures. The effect of the macroelement composition from three types of medium: MS (Murashige and Skoog), QL (Quoirin and Lepoivre), and DKW (Driver and Kuniyuki Walnut) was checked, while the microelements and vitamins in all the treatments were according to the MS recipe. The effect of four concentrations (0.1, 0.5, 1.0, 2.0 mg·L–1) of cytokinins: benzyladenine (BA) and meta-Topolin (mT) on the number and length of shoots was evaluated. Also the effect of different concentrations of auxins: IBA (indole-3-butyric acid) or NAA (1-naphthylacetic acid) on the percentage of rooted cuttings as well as the number and length of roots was determined. The last culture phase was the acclimatization in a greenhouse. The media and the growth regulators significantly affected the rate of propagation in the coral bells. The highest shoot number was obtained on the modified QL with BA, while NAA gave better results of rooting than IBA. Optimal conditions for micropropagation allow to obtain approx. 10,000 microplants from a single explant during the three months of culture.
Keywords
in vitro; micropropagation protocol; parennials; coral bells; cytokinins; auxins
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